Construct:Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: For mEPSCs, events were analyzed by Mini Analysis Program software (Synaptosoft, Inc., Decatur, GA). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Plasmid Preparation:Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: For mEPSCs, events were analyzed by Mini Analysis Program software (Synaptosoft, Inc., Decatur, GA). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Sequencing:Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: For mEPSCs, events were analyzed by Mini Analysis Program software (Synaptosoft, Inc., Decatur, GA). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Control:Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: For mEPSCs, events were analyzed by Mini Analysis Program software (Synaptosoft, Inc., Decatur, GA). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Transfection:Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: For mEPSCs, events were analyzed by Mini Analysis Program software (Synaptosoft, Inc., Decatur, GA). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. RNAi constructs The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
Article Title: GLUTAMATERGIC SYNAPSE FORMATION IS PROMOTED BY ?7-CONTAINING NICOTINIC ACETYLCHOLINE RECEPTORS
Article Snippet: .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine TM 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio). .. The β2-RNAi construct (generously provided by Uwe Maskos; Avale et al., 2008 ) targeting the mouse β2-nAChR transcript was tested for effectiveness by using Lipofectamine 2000 (Life Technologies, Grand Island, NY) to transfect HEK293T cells on glass coverslips with plasmids encoding α4-nAChR subunit from chicken, cyan fluorescent protein (CFP)-tagged β2-nAChR subunit from mouse (Addgene plasmid 15106, developed by H. Lester, Caltech), and GFP together with either β2-RNAi or a scrambled version of the sequence (β2-Scr) as nonspecific control (1:1:3 transfection ratio).
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